Involved in investigating the effects of antioxidants from fruit extracts on the growth of cancer cells in culture. The fruit extracts have been separated into anthocyanin- and ellagitannin-rich fractions and their antiproliferative effects tested on the growth of HeLa cells.
Further investigation on colon cancer cells will be carried out using the Transwell system so uptake and conversion of antioxidants by a cell monolayer can be measured. Extracts from a range of different fruits have been tested and the fractions having the highest antiproliferative effect evaluated.
A new area of my current work is to develop techniques for measuring potato texture.
Many years' experience in potato physiology and biochemistry. Investigation of sucrose cleaving enzymes in the metabolism of a range of storage organs formed the study for my PhD.
Later work involved protein separation techniques and protein identification by Edman sequencing and led to the identification of unknown proteins from strawberries.
Involved in the production of a range of transgenic potato plants to investigate metabolic effects of differentially expressing genes coding for enzymes involved in carbohydrate and ascorbate metabolism.
Studied the enzyme Limit Dextrinase in malting Barley grains.
Ross, H.A., McDougall, G.J., Stewart, D. 2007. Antiproliferative activity is predominantly associated with ellagitannins in raspberry extracts. Phytochemistry 68, 218-228.
Souleyre, E., Taylor, M.A., Iannetta, P.P.M., Ross, H.A., Shepherd, L.V.T., Hancock, R.D., Viola, R. and Davies, H. V. 2004. Starch metabolism in developing strawberry (Fragaria x ananassa) fruits. Physiologia Plantarum 121, 369 - 276.
McDougall, G.J., Ross, H.A., Swanston, J.S. and Davies, H.V. 2004. Limit dextrinase from germinating barley has endotransglycosylase activity which explains its activation by linear maltodextrins. Planta 218, 542 - 551.
Iannetta, P.P.M., Medina Escobar, N., Ross, H.A., Souleyre, E.J.F., Hancock, R.D., Witte, C.P. and Davies, H.V. 2004. Identification, cloning and expression analysis of strawberry (Fragaria x ananassa) Duch. mitochondrial citrate synthase and mitochondrial malate dehydrogenase. Physiologia Plantarum 121, 15 - 26.
McDougall, G.J., Ross, H.A., Swanston, J.S. and Davies, H.V. 2004. A novel activity for a starch debranching enzyme. Annual Report 2003/2004, Scottish Crop Research Institute, Invergowrie, Dundee, 142 - 143.
Di Matteo, A., Hancock, R.D., Ross, H.A., Frusciante, L. and Viola, R. 2003. Characterisation of Chlorella pyrenoidosa L-ascorbic acid accumulating mutants: Identification of an enhanced biosynthetic enzyme activity and cloning of the putative gene from Arabidopsis thaliana. Proceedings of SEB 2003, Southampton. Comparative Physiology and Biochemistry134, S155 (Poster).
Ross, H.A., Sungurtas, J., Ducreux, L., Swanston, J.S., Davies, H.V. and McDougall, G.J. 2003. Limit dextrinase in barley cultivars of differing malting quality: activity, inhibitors and limit dextrin profiles. Journal of Cereal Science38, 325 - 334.
Viola, R., Hancock, R.D., Ross, H.A., Di Matteo, A. and Simpson, C.G. 2003. Nucleotide pyrophosphatase gene sequence.
Taylor, M.A., Ross, H.A., McRae, D., Wright, F., Viola, R. and Davies, H.V. 2001. Copy-DNA cloning and characterisation of a potato alpha-glucosidase: expression in Esherichia coli and effects of down-regulation in transgenic potato. Planta213, 258 - 264.
Iannetta, P.P.M., Medina-Escobar, N., Ross, H.A., Souleyre, E., Hancock, R.D., Fairlie, J., Taylor, M.A. and Davies, H.V. 2000. Enzymes of the TCA cycle expressed during strawberry fruit ripening. Abstracts of the 4th International Strawberry Symposium. Tampere, Finland, 9-14 July 2000.
Iannetta, P.P.M., Medina-Escobar, N., Ross, H.A., Souleyre, E., Taylor, M.A. and Davies, H.V. 2000. Enzymes of the TCA cycle expressed in strawberry fruit during ripening. The Scottish Cell and Molecular Biology Group. St Andrews University, 28 January 2000.
Taylor, M.A., Ross, H.A., McRae, D., Stewart, D., Roberts, I.M., Duncan, G.H., Wright, F., Millam, S. and Davies, H.V. 2000. A potato α-glucosidase gene encodes a glycoprotein-processing α-glucosidase II-like activity. Demonstration of enzyme activity and effects of down-regulation in transgenic plants. Plant Journal24, 305 - 316.
Ziegler, A., Cowan, G.H., Torrance, L., Ross, H.A. and Davies, H.V. 2000. Facile assessment of cDNA constructs for expression of functional antibodies in plants using the Potato virus X vector. Molecular Breeding 6, 327 - 335.